Runs 294
Samples 27,295
Unique samples 22,209
Total processed reads 344,959,688,225
Total valid reads 275,569,397,993
Total reads in unique samples 217,384,391,197

Used databases

Name Version
miRBase 22.1
Ensembl ncRNA 100
RNACentral piRNA 15
GtRNAdb 18.1
circBase Accessed 25.10.20
(bacteria & viruses)


  • 25.10.2020: Release of version 2.0.0-rc. We added support for 7 other organisms, custom and UMI-based sequencing protocols, circular RNAs and several new analysis modules.

miRMaster 2 is a comprehensive analysis framework for sncRNA-seq data. Samples are directly preprocessed in the browser before being uploaded to our server.

Our tool quantifies miRNAs and other ncRNAs, as well as isomiRs and variants in miRNAs and predicts miRNA candidates.

Further it performs mapping against the NCBI RefSeq collection of bacterial and viral genomes, thereby allowing the detection of contaminations, infections or exogenous miRNAs.

We also provide correlation analyses, clustering and embedding analyses, as well as batch effect quantification and differential expression evaluation.


If you used miRMaster in your work please cite:

T. Fehlmann, C. Backes, M. Kahraman, J. Haas, N. Ludwig, A. E. Posch, M. L. Würstle, M. Hübenthal, A. Franke, B. Meder, E. Meese, A. Keller; Web-based NGS data analysis using miRMaster: a large-scale meta-analysis of human miRNAs. Nucleic Acids Res 2017 gkx595. doi: 10.1093/nar/gkx595

Browser compatibility
Our web server supports a variety of common operating systems and browsers:
OS Version Chrome Firefox Microsoft Edge Safari
Linux Manjaro (64 bit) 20.0.1 87.0.4280.88 83.0 N/A N/A
macOS Mojave 10.14.6 85.0.4183.83 83.0 N/A 14.0.2
Windows 10 85.0.4183.83 83.0 87.0.664.41 N/A

For the best experience we recommend to use either Chrome or Firefox.